Category Archives: pub_2024

Matrix and analysis metadata standards (MAMS) to facilitate harmonization and reproducibility of single-cell data

Irzam Sarfraz, Yichen Wang, Amulya Shastry, Wei Kheng Teh, Artem Sokolov, Brian R. Herb, Heather H. Creasy, Isaac Virshup, Ruben Dries, Kylee Degatano, Anup Mahurkar, Daniel J. Schnell, Pedro Madrigal, Jason Hilton, Nils Gehlenborg, Timothy Tickle & Joshua D. Campbell  Genome Biology volume 25, Article number: 205 (2024) Cite this article 236 Accesses 5 Altmetric Metricsdetails Abstract Many datasets are being produced by consortia that seek to characterize healthy and disease tissues at single-cell resolution. While biospecimen and experimental information ……

Characterization and decontamination of background noise in droplet-based single-cell protein expression data with DecontPro

Yuan Yin 1, Masanao Yajima 2, Joshua D Campbell 1 Affiliations Expand Abstract Assays such as CITE-seq can measure the abundance of cell surface proteins on individual cells using antibody derived tags (ADTs). However, many ADTs have high levels of background noise that can obfuscate down-stream analyses. In an exploratory analysis of PBMC datasets, we find that some droplets that were ……